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Environmental Factor

Environmental Factor

Your Online Source for NIEHS News

November 2020

Breast cancer and air pollution linked by new marker

NIEHS scientists connect particulate matter exposures with tissue changes linked to increased breast cancer risk.

NIEHS researchers and colleagues uncovered a previously unknown connection between outdoor air pollution exposure and a breast tissue marker related to increased breast cancer risk. The findings were published Sept. 24 in the journal Breast Cancer Research.

Alexandra White, Ph.D. White completed a postdoctoral fellowship in the NIEHS Epidemiology Branch and joined the branch as an Earl Stadtman tenure-track investigator in 2019. (Photo courtesy of Steve McCaw)

In the first study of its type, the scientists cross-referenced the tissue marker with air pollution data for the tissue donors’ addresses, in a manner that protected the privacy of the donors. Alexandra White, Ph.D., head of the NIEHS Environment and Cancer Epidemiology Group, was the senior author. Postdoctoral fellow Nicole Niehoff, Ph.D., was first author of the paper.

The researchers analyzed more than 1,900 samples of breast tissue from the Komen Tissue Bank (see sidebar). They evaluated an anatomical feature called terminal duct lobular units (TDLUs), which are the site in the breast where most breast cancers originate.

As women age, their TDLUs naturally shrink and become fewer in number. This process is called involution. Other researchers have linked reduced involution — when TDLUs fail to shrink in number and size — to increased risk of breast cancer.

Mapping exposures

“In this population, we geocoded the participants’ addresses,” said Niehoff, first author of the paper. “Using a publicly available EPA [U.S. Environmental Protection Agency] database, we linked air pollution levels at the census tract level with each tissue sample.”

The team compared number and size of TDLUs with air pollution exposure levels. Among the air pollution components that the researchers evaluated, living in areas with high levels of fine particulate matter, which is defined as particles smaller than 2.5 micrometers, or PM2.5, proved to be the strongest association. Those tissue samples showed reduced involution, and therefore potentially a higher risk of developing breast cancer later on.

steel mills in Indiana In addition to PM2.5, the team reported gaseous pollutants were associated with elevated TDLU count, but not in a linear fashion. Shown here, steel mills in Indiana.

New research territory

White, the paper’s senior author, found connecting the biomarker with exposure data to be a rich line of research, adding to her research group’s earlier work. “There’s been very little research on how environmental exposures might influence TDLUs,” she said. The challenge of obtaining samples of normal tissue is one hurdle researchers must overcome.

Nicole Niehoff, Ph.D. Niehoff worked on the statistical analyses for the project and wrote the first draft of the manuscript. (Photo courtesy of Steve McCaw)

Niehoff noted that the research forges new territory. “Establishing these tissue-wide changes is a new contribution to the literature and provides additional evidence for continuing to look at the association between air pollution and breast cancer,” she said. “Our findings strengthen the biologic rationale for the connection between air pollution and breast cancer.”

The scientists noted that although they do not know the mechanism by which PM2.5 influences changes in breast tissue, some researchers suspect that it disrupts hormone balances. The authors cited studies suggesting that TDLU involution may occur at least in part through a hormone-related pathway.

Moving on to metals

White’s team is excited about extending their work on TDLUs. “We are also looking at exposures to other airborne pollutants, with Nikki [Niehoff] leading some work on airborne metals,” said White. Metals that can become airborne and affect health include lead, cadmium, and mercury.

She also praised the contributions of her co-authors from the National Cancer Institute’s Division of Cancer Epidemiology and Genetics. “This is a great example of NIH wide collaboration,” she said.

Citation: Niehoff NM, Keil AP, Jones RR, Fan S, Gierach GL, White AJ. 2020. Outdoor air pollution and terminal duct lobular involution of the normal breast. Breast Cancer Res 22(1):100.

(Ernie Hood is a contract writer for the NIEHS Office of Communications and Public Liaison.)

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